| 1. | Integration through homologous recombination between the 2 . 7 kb pks gene and the natural pks gene was confirmed by southern hybridization
Southern杂交证实2 . 7kbpks基因和天然pks基因之间通过同源重组发生了整合。 |
| 2. | Rt - pcr combined southern hybridization analysis show that hap2 is expressed in leaves , tepals , and tepals , stamens of floral buds
Rt pcr结合southern杂交分析表明， hapz在叶、花被片、再生花芽的花被片和雄蕊中均表达。 |
| 3. | ( 2 ) it proved that there should be nucleotides sequence similar to mf - 14 in the sterile line by the southern hybridization using the fragment mf - 14 as probe
( 2 )以回收得到特异片段mf - 14为探针，通过southern杂交检测到白菜不育系中也含有该片段的同源序列。 |
| 4. | ( 3 ) because the expression signal of map2a was hard to be detected by the northern hybridization , we combined rt - pcr and southern hybridization to determine its expression
( 3 )鉴于map2a基因的表达量较低，我们采用rt - pcr结合southern杂交的方法，研究了map2a基因在苹果不同组织中的表达状况。 |
| 5. | Strain sa - coo by southern hybridization . a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357 , a streptomyces - e . coli bifunctional vector carrying two cohesive sites
为了获得胆固醇氧化酶基因，以大肠杆菌-链霉菌双功能柯斯质粒phz1357为载体，构建了灰色链霉菌atcc14811的基因组文库。 |
| 6. | As heterologous probe and subsequently show to code for desired enzymatic activity . after a serial of subcloning coupled with southern hybridization and enzymatic activity assay , the functional s . griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2 . 3kb ecori - sall fragment
对phz1140和phz1141进行bamh及bgl的酶谱分析及与choa探针的杂交，将胆同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。 |
| 7. | It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent , genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study , genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study , a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha , further confirming the veracity of the results from eric - pcr ; 7 in this study , isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳属2个种的2个菌株在its区域表现出较高的保守性， 4种限制型内切酶的酶切图谱没有显示出多态性；增加内切酶种类及供试菌株数量，有可能获得具有多态性的限制性内切酶酶切图谱； 9本实验中， its区域的真菌特异性引物与真核生物通用引物对于扩增效果无较大差异，扩增片段长度均为650bp左右； 10根据形态学实验、 eric - pcr实验以及southern杂交实验的结果分析，紫木木耳属种质资源的遗传鉴定和遗传多样性评价耳极有可能是毛木耳种的一个变种; n .本研究中所用的gutc法是一种适用于木耳属菌株基因组洲a快速提取的方法; 12 .传统的形态学分类法和现代的分子生物学分类法，两者的关系是相辅相成，互为验证 |
| 8. | According to the southern hybridization results , 43 bacillus strains were divided into several groups , 22 strains were chosen randomly from all these groups for sequencing of aii gene . it was found that the nucleotide acid sequence of aii genes showed 85 . 4 % - 100 % homology and amino acid sequence of aii proteins showed 8 8 . 1 % - 100 % ho mology
在southern杂交的基础上，将检测的43个菌株分组，对其中22个菌株的aii基因进行dna序列测定，结果表明，不同菌株aii基因的dna序列同源性为85 . 4 ? 100 ；而aii蛋白的氨基酸序列同源性为88 . 1 ? 100 。 |
| 9. | Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak . 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells . the homologous recombination occurred inside the cells , and the recombinant virus bacpak - 6aa - hgm - csf was expressed , as identified by pcr and southern hybridization . the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf
本研究首先通过pcr将家蚕杆状病毒多角体蛋白起始密码子后的18个碱基引入到hgm - csf基因的5 ’端之前，然后将融合基因重组与家蚕杆状病毒转移载体pbacpak8中，获得重组转移载体pbacpak8 - 6aa - hgm - csf ，并与线性化bm - bacpak6dna共转染家蚕细胞株，获得重组病毒bacpak - 6aa - hgm - csf 。 |
